《植物生理学报》 2009, 45(7): 663-666

芸薹属中几个物种碳酸酐酶活性的比较

邓秋红, 甘露, 付春华, 栗茂腾*, 余龙江*

华中科技大学生命科学与技术学院, 武汉430074

通信作者：栗茂腾；E-mail: yulongjiang@mail.hust.edu.cn, limaoteng426@163.com；Tel: 027-87792264

摘　要：

检测芸薹属中几种植物的碳酸酐酶在根、茎、叶中的分布和活性以及叶片净光合速率和锌元素含量的结果表明: 碳酸酐酶在甘蓝型油菜的根、茎、叶中均有分布, 叶中的碳酸酐酶活性最大, 茎中的次之, 根中的最小。不同芸薹属植物中 碳酸酐酶活性的大小依序为: 甘蓝型油菜>白菜型油菜>芜菁>埃塞俄比亚芥。以甘蓝型油菜为例, 碳酸酐酶活性的日变化 呈双峰曲线, 在整个生育期的变化趋势是先上升后下降, 抽薹期间的碳酸酐酶活性最高。碳酸酐酶活性与净光合速率以及 与锌元素含量之间均呈现显著的正相关。

关键词：芸薹属; 甘蓝型油菜; 碳酸酐酶活性; 净光合速率; 锌含量

收稿：2009-03-30   修定：2009-05-20

资助：国家“863” 计划(2009AA101105)和华中科技大学研究基金(2006Q056B)。

Comparison of Carbonic Anhydrase Activities of Several Species in Brassica

DENG Qiu-Hong, GAN Lu, FU Chun-Hua, LI Mao-Teng*, YU Long-Jiang*

College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China

Corresponding author: LI Mao-Teng; E-mail: yulongjiang@mail.hust.edu.cn, limaoteng426@163.com; Tel: 027-87792264

Abstract:

The distribution and activities of carbonic anhydrases (CA) in root, stem, and leaf of several species in Brassica were studied in order to determine the correlations between CA activity with net photosynthetic rate or zinc content. It was showed that CAs were distributed extensively in root, stem, and leaf of Brassica. CA activity was much higher in leaves than that in roots or stems, while enzyme activity in roots was the lowest. Considering different species in Brassica, CA activities in leaves were descending with the range as B. napus L., B. campestris L., B. rapa L., and B. carinata L. The diurnal variation of CA activity in B. napus leaves showed the double-peak curve during whole growth period, which reached its maximum at the bolting stage. It could be concluded that CA activity had positive correlation with net photosynthetic rate and zinc content, respectively.

Key words: Brassica; Brassica napus; carbonic anhydrase activity; net photosynthetic rate; zinc content